Uterine morphogenesis and dynamics of the cytokine profile of the blood plasma of the reproductive period rats
at correcting of methotrexate-induced immunosuppression with imunofan 

Sergey N. Semenchuk, Candidate of medical sciences, associate professor of the sub-department of histology, cytology and embryology, Saint Luka Lugansk State Medical University (1g 50-letiya Oborony Luganska square, Lugansk, Russia), E-mail: s.semenchuk.lgmu@mail.ru
Svetlana A. Kashchenko, Doctor of medical sciences, professor, head of the sub-department of histology, cytology and embryology, Saint Luka Lugansk State Medical University (1g 50-letiya Oborony Luganska square, Lugansk, Russia), E-mail: kashchenko_s@list.ru 

Background. The purpose of the work is to study uterine morphogenesis and dynamics of the cytokine profile of the blood plasma of the reproductive period rats at correcting of methotrexate-induced immunosuppression with imunofan. Materials and methods. The study was carried out on 60 white mongrel mature female rats weighing 210-250 g. After synchronizing the estrous cycles, the animals were divided into 2 groups. Immunosuppression was modeled by administration of methotrexate to laboratory animals at a dosage of 10 mg/m2 of body area at the beginning of diestrus, followed by correction by intramuscular injection of the immunomodulatory agent imunofan at a dosage of 50 μg according to the scheme. Rats were taken out of the experiment into the
proestrus phase (PE) of the 1st, 3rd, 5th, 7th and 9th estrous cycles, the absolute and relative mass of the uterus, the linear characteristics of its body and horns, and the volume of the organ were measured. Micromorphometric parameters were determined at the lightoptical level. To establish the characteristics of the state of the immune system, the content of mediators of intercellular interaction IL-1β, IL-2, IL-6 and TNFa in the blood plasma of animals was determined. Results. It was found out that the studied influence has a pronounced effect on the morphometric parameters of the uterus of white mongrel female rats of young reproductive age, and is also accompanied by statistically significant changes in the cytokine profile of animals. Conclusions. The results obtained indicate a favorable effect of imunofan on the dynamics of morphometric parameters of the uterus of mature animals as a corrector of acute immunosuppression. 

methotrexate, imunofan, uterus, immunosuppression, rats 

Semenchuk S.N., Kashchenko S.A. Uterine morphogenesis and dynamics of the cytokine profile of the blood plasma of the reproductive period rats at correcting of methotrexate-induced immunosuppression with imunofan. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskie nauki = University proceedings. Volga region. Medical sciences. 2022;(4):66–73. (In Russ.). doi:10.21685/2072-3032-2022-4-7